Antimicrobial Resistance, Multilocus Sequence, and spa Typing of Staphylococcus aureus Isolated from Retail Raw Meat Products

With a high capacity to acquire antimicrobial resistance, Staphylococcus aureus is an important pathogen causing severe infections in animals and humans. A total of 50 isolates from retail ground beef, chicken meat, fish were characterized by resistance profiling, staphylococcal protein gene (spa) typing, multilocus sequence typing (MLST). The broth microdilution test results showed that all resistant penicillin sulphamethoxazole but had varying rates tetracycline (24%), erythromycin (4%), gentamicin (2%), ciprofloxacin trimethoprim chloramphenicol (0%). blaZ sulI genes detected 100% the followed grlA (94%), norA (92%), tetK (80%), chlA (60%), tetM (26%), aacA-aphD ermA fexA (0%), dfrA Moreover, 26% multidrug-resistant, with five or more genes. spa analysis revealed 22 types, t091 (16%), t1677 (8%), t14538 (8%) being most common, one new type, t19851, was uncovered. MLST identified seven types (STs), ST7 (40%), ST15 (20%), ST199 (13%) two STs (ST7435 ST7436) newly identified. In this study, S. isolated raw meat multidrug different clones associated human infections. As result, foods animal origin may act as potential vehicles for transmission multidrug-resistant isolates, dissemination potentially pathogenic clonal posing health risk